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Handy PEA 連續激發(fā)式熒光儀
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Handy PEA植物效率分析儀 能夠完整測定葉綠素OJIP熒光誘導動(dòng)力學(xué)曲線(xiàn)的連續激發(fā)式熒光儀 主要應用范圍: 植物生理學(xué)、植物生態(tài)學(xué)、農學(xué)、園藝學(xué)、林學(xué)、環(huán)境科學(xué)、植物病理學(xué)、藻類(lèi)生物學(xué)等領(lǐng)域。 測定參數: 全新軟件可直接計算出Fo,Fm,Fv,Fv/Fm,Ft,FJ,FI,FP,Tm, ψo,φEo,φDo,Vt,VJ,WK,PIABS,PICS,ABS/RC,TRo/RC,ETo/RC,DIo/RC,RC/CSo,RC/CSM等五十個(gè)葉綠素熒光參數。準確記錄葉綠素熒光誘導動(dòng)力學(xué)曲線(xiàn)的快相部分,每秒鐘可以連續記錄10萬(wàn)次熒光蹤跡數據,在1秒鐘內完整測定葉綠素的OJIP熒光誘導動(dòng)力學(xué)曲線(xiàn)。 全新的葉綠素熒光理論詮釋 Handy PEA植物效率分析是葉綠素熒光儀的一種,屬于連續激發(fā)式熒光儀。Handy PEA是PEA植物效率分析儀系列儀器中功能強大,價(jià)格中等,國內用戶(hù)數量非常龐大。 與傳統的脈沖調制式熒光儀不同,Handy PEA具有超高的時(shí)間分辨率,分辨率高達105,是能夠準確記錄O-J-I-P快速葉綠素熒光誘導動(dòng)力學(xué)曲線(xiàn)的儀器。幾乎所有植物(>99%)的OJIP曲線(xiàn)可以在1秒鐘內完成準確測定。OJIP曲線(xiàn)的數據分析使用基于JIP-Test的分析方法,該方法由PEA植物效率分析儀的創(chuàng )始人Strasser教授創(chuàng )建。 通過(guò)OJIP曲線(xiàn)我們可以準確的分析光合機構在逆境下,是否受到傷害?光合機構哪個(gè)部位受到傷害?受傷害的程度多大?既可以進(jìn)行定性分析,也可以對傷害程度進(jìn)行定量分析。 儀器發(fā)明人Strasser教授簡(jiǎn)介: R.T.Strasser是瑞士日內瓦大學(xué)教授,國際禁用詞匯的光合作用研究專(zhuān)家,是日內瓦大學(xué)生物能學(xué)和微生物學(xué)重點(diǎn)實(shí)驗室的主任,聯(lián)合國地中海沙漠植被恢復項目評估專(zhuān)家組成成員,主要從事光合作用和光合模擬研究。 高等植物,藻類(lèi)通用的熒光儀 圖5 用于高等植物的Handy PEA探頭 圖6 用于藻類(lèi)樣品的Handy PEA探頭 Handy PEA植物效率分析儀,標配高等植物探頭,非常方便的進(jìn)行葉片O-J-I-P快速葉綠素熒光誘導動(dòng)力學(xué)曲線(xiàn)的測定,也可以選配藻類(lèi)樣品探頭,測定藻類(lèi)的OJIP曲線(xiàn)。 測定高等植物的探頭測定不受葉面積的影響,HPEA/LC暗適應夾(葉夾),測定直徑為4mm,對于小于4mm的植物葉片,Handy PEA通過(guò)數據標準化進(jìn)行處理,不影響數據的準確性。 測定藻類(lèi)的樣品探頭HPEA/LPA2具有極高的分辨率,很低的藻類(lèi)樣品即可進(jìn)行準確的測定,如硅藻,在肉眼幾乎分辨不出存在的情況下,即可測定完整準確的OJIP曲線(xiàn)。 超級便攜的用戶(hù)體驗 圖7 便攜性?xún)?yōu)異的Handy PEA用于田間測定 Handy PEA植物效率分析儀全套設備重量不足1kg,有背帶,方便進(jìn)行野外操作,即使是女科研人員也可以一個(gè)人完成所有的操作。 測定數據快速,儀器標配20個(gè)暗適應夾(6g),可以一次進(jìn)行至少20個(gè)樣品的暗適應處理,測定一個(gè)樣品的時(shí)間為2s左右?梢栽跇O短的時(shí)間內完成大量的測定,獲取大量的有價(jià)值的數據。在大數據時(shí)代,Handy PEA必然會(huì )進(jìn)一步體現出它的強大功能。 功能強大的軟件
圖 8 友好的Handy PEA軟件界面 軟件界面友好,操作簡(jiǎn)單,具有篩選的功能,可以在大數據時(shí)代,用于優(yōu)良品種、抗性品種的篩選。進(jìn)行分組、分類(lèi)、平均、規律等分析。 強大的售后服務(wù)團隊 在駱兵博士帶領(lǐng)的所有的售后服務(wù)工程師都具有碩士研究生及其以上學(xué)歷,并具有使用該儀器做實(shí)驗3年以上的實(shí)戰經(jīng)驗。 售后服務(wù)既可以提供儀器的理論講座,也可以專(zhuān)門(mén)介紹在儀器使用過(guò)程中可能出現的錯誤及避免方案,還可以介紹大量的寶貴經(jīng)驗,或者實(shí)測用戶(hù)現有的材料,進(jìn)行數據分析,幫助用戶(hù)在短時(shí)間內將儀器用于科研工作。 數量巨大的文獻支持(禁用詞匯新文獻請查看微信公眾號“漢莎科技集團”) 2015年 Agrawal D, Jajoo A. Investigating primary sites of damage in photosystem II in response to high temperature[J]. Indian Journal of Plant Physiology, 2015, 20(4): 304-309. Akhtar S S, Andersen M N, Liu F. Residual effects of biochar on improving growth, physiology and yield of wheat under salt stress[J]. Agricultural Water Management, 2015, 158: 61-68. Akhter A, Hage-Ahmed K, Soja G, et al. Compost and biochar alter mycorrhization, tomato root exudation, and development of Fusarium oxysporum f. sp. lycopersici[J]. Frontiers in plant science, 2015, 6: 529. Amini F, Ramin A A. 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